The Third European Workshop on Cell Death

Salobreña, Spain

February 23rd to 28th 2002

PROGRAMME

Saturday February 23^rd

 15:00 - 19:00          Registration in Hotel Salobreña

 19:00                        Welcome reception in Salobreña town

 21:00                        Dinner in Hotel Salobreña

Sunday  February 24^th

 8:00 - 9:00       Breakfast

 9:30 - 11:15   Expression and processing of TNF family members

                                             Chair: Michel Hahne, Giorgio Stassi

Kerstin Herzer                      MECHANISM OF TGF-ß INDUCED APOPTOSIS IN HEPATOMA CELLS

Anne Grosse-Wilde              BIOCHEMICAL ANALYSIS OF MURINE TRAIL RECEPTORS

Raymond Daniels                 TRAIL and TRAIL receptor expression in normal and malignant tissues.

Carmen Ruiz de Almodóvar UP-REGULATION OF TRAIL RECEPTORS IN BREAST TUMOR CELLS UPON GENOTOXIC TREATMENT

Marta López Fraga                INTRACELLULAR PROCESSING OF APRIL, A TNF LIGAND FAMILY MEMBER

Marcello Pinti                       GENETIC POLYMORPHISMS OF FAS (CD95) AND FASL  (CD178) IN HUMAN LONGEVITY: STUDIES ON CENTENARIANS

11:15 - 11:45           Coffee break

11:45 - 13:00          TNF like ligands: Biology and novel signalling

                                                      Chair: Giorgio Stassi, Michel Hahne

Desiree Bonci                      APRIL IS AN AUTOCRINE GROWTH FACTOR FOR MEGAKARYOCYTES

Alena Vaculová                   Tumour necrosis factor-alpha-induced cell death in colon cancer cells

Wiebke Baum                      IDENTIFICATION OF PROTEINS INTERACTING WITH THE INTRACELLULAR DOMAIN OF CD95LIGAND  

Christoph Wasem                 REVERSE SIGNALLING VIA DEATH LIGANDS OF THE TUMOR NECROSIS FACTOR SUPERFAMILY

13:00 - 15:00           Lunch

15:00 - 16:45     Assembly and inhibition of signalling complexes 

                                Chair: Henning Walczak, Ruggero De Maria

Martin Sprick                Caspase-10 is recruited to and activated at death-inducing signalling complexes in a FADD-dependent manner

Tom Ganten                 Enhanced Caspase-8 Recruitment to the DISC is Critical for Sensitisation of Human Hepatoma cells to TRAIL-induced Apoptosis BY CHeMOTHERAPEUTIC DRUGS  

Fabio Martinon             THE INFLAMMOSOME - A NOVEL SIGNALLING COMPLEX INVOLVED IN INFLAMMATORY CASPASE ACTIVATION

Adriana Eramo              CD95 SIGNALLING IS INITIATED IN LIPID RAFTS

Francesco Lozupone    CD95/EZRIN INTERACTION IN THE CD95 APOPTOTIC PATHWAY

Andreas Krueger           c-FLIP splice variants inhibit different steps of caspase-8 activation at the CD95 death-inducing signaling complex (DISC)

16:45 - 17:15           Coffee break

17:15 - 18:30        Regulation of cell death 1: at receptor level 

                                       Chair: Ruggero De Maria, Henning Walczak

Kristina Archer              MECHANISM OF DECOY RECEPTOR REGULATION OF TRAIL CYTOTOXICITY IN LYMPHOCYTES

Astrid Ruefli                  Mechanisms of P-glycoprotein-mediated inhibition of Fas-induced cell death  

Cristina Muñoz-Pinedo  Inhibition of glucose metabolism sensitizes tumor cells to apoptosis induced by TRAIL, cd95 antibodies and tnf

Olivier Disson                 REGULATION OF HEPATOCYTE APOPTOSIS IN A TRANSGENIC MODEL OF HCV INFECTION

20:00                         Dinner

Monday February 25^th

  8:00 - 9:00                Breakfast

  9:15 - 11:00           Regulation of cell death 2: by Bcl-2 family proteins

                                            Chair: Jan Paul Medema, Marion MacFarlane

Andreas Villunger           BMF: A PROAPOPTOTIC BH3-ONLY PROTEIN REGULATED BY INTERACTION WITH THE MYOSIN V ACTIN MOTOR COMPLEX, ACTIVATED BY ANOIKIS

Arlette Werner                 DEFINING THE MECHANISM OF ACTION OF BFL-1,  A NOVEL INHIBITOR OF BID

Pilar Justo                       MODULATION OF ACETAMINOPHEN INDUCED APOPTOSIS IN CULTURED MURINE TUBULAR EPITHELIAL CELLS BY BclxL AND INHIBITORS OF CASPASES

Paul Coffer                      THE FORKHEAD TRANSCRIPTION FACTOR FKHR-L1 IS A CRITICAL EFFECTOR OF CELL DEATH INDUCED BY SURVIVAL FACTOR WITHDRAWAL

Barbara Marinari             Vav exchange factor blockS CD4-mediated signals, inducing Bax increase and mitochondrial damage

Penny  Lovat                   GADD153 AND BAK:NOVEL MOLECULAR TARGETS OF FENRETINIDE-INDUCED APOPTOSIS

11:00 - 11:30             Coffee break

11:45 - 13:15          Regulation of cell death 3: at mitochondrial level and beyond

                                    Chair: Marion MacFarlane, Jan Paul Medema

Nigel Waterhouse             Single cell analysis of reactive oxygen species during mitochondrial outer membrane permeabiliSation

Geert Van Loo                  Identification of tBid-induced release of mitochondrial proteins. Role of endonuclease G and Omi/HtrA2 in the apoptotic process

Marion MacFarlane           TRAIL-INDUCED APOPTOSIS RESULTS IN RAPID RELEASE OF SMAC AND ITS SUBSEQUENT DEGRADATION VIA THE UBIQUITIN-PROTEIN LIGASE ACTIVITY OF XIAP

 John Silke                          THE ROLE OF XIAP AND DIABLO/SMAC IN APOPTOSIS

Marie-Louise Brezniceanu  IDENTIFICATION OF MAMMALIAN PROTEINS INHIBITING APOPTOSIS DOWNSTREAM OF CYTOCHROME C RELEASE

Klaus Schulze-Osthoff       Ionizing radiation but not cancer drugs causes G2/M cell cycle arrest and failure to activate the apoptotic pathway in breast carcinoma cells

13:15 - 15:00           Lunch

15:00 - 16:45             Regulation of cell death 4: Novel mechanisms

                                        Chair: Eric Baehrecke, Nigel Waterhouse

Gerolama Condorelli       MULTIPLE MEMBERS OF THE MITOGEN ACTIVATED PROTEIN KINASE FAMILY ARE NECESSARY FOR PED/PEA-15 ANTI-APOPTOTIC FUNCTION

Martin Leverkus               RESISTANCE TO TRAIL INDUCED APOPTOSIS IN PRIMARY KERATINOCYTES: A ROLE FOR EFFECTOR CASPASE INHIBITION

Grisha Pirianov               Rac is Involved in the Apoptotic Response of N1E-115 Neuroblastoma Cells to Staurosporine, but not to Ceramide

Eric Baehrecke               GENETIC REGULATION OF PROGRAMMED CELL DEATH DURING DROSOPHILA DEVELOPMENT

Arturo Sala                      UNVEILING THE APOLIPOPROTEIN J SIGNALLING NETWORK: IMPLICATIONS FOR CELL SURVIVAL, PROLIFERATION AND ONCOGENESIS

Maria Fiammetta Romano  A.I.R. (APOPTOSIS-INDUCED AND REGULATOR), A NOVEL GENE THAT REGULATES APOPTOSIS

16.45 - 19.45         Poster Session with drinks and tapas sponsored by

20:00                         Dinner

Tuesday February 26^th

8:00 - 9:00                Breakfast

9:15 - 11:00           Regulation of cell death 5: extracellular factors                                 

                                Chair: Vincenzo De Laurenzi, Cristina Muñoz-Pinedo

Matilde Todaro                Th1and Th2 cytokines control cell survival in thyroid cancer by modulating apoptotic proteins

Concetta Conticello         INTERLEUKIN-4 PROTECTS TUMOR CELLS FROM CD178 AND CHEMOTHERAPIC AGENTS VIA UPREGULATION OF ANTIAPOPTOTIC PROTEINS

Igor Cima                        IN SITU PRODUCTION OF GLUCOCORTICOIDS IN THE INTESTINE AS A REGULATOR OF LIFE AND DEATH IN INTESTINAL T-LYMPHOCYTES

Eric Eldering                   MITOCHONDRIAL UNCOUPLING ACTIVATES CASPASE-9 AND MIMICS ANTIGEN RECEPTOR- INDUCED APOPTOSIS IN B-CELLS

Ann Zeuner                   STEM CELL FACTOR UPREGULATES BCL-2 FAMILY MEMBERS AND PROTECTS ERYTHROID PRECURSOR CELLS FROM CHEMOTHERAPY-INDUCED APOPTOSIS

Anastasis Stephanou      RELATIVE CONTRIBUTION OF THE MITOCHONDRIA AND DEATH RECEPTOR SIGNALING PATHWAYS IN CARDIAC APOPTOSIS DURING ISCHAEMIA VERSUS REPERFUSION INJURY

  11:00 - 11:30            Coffee break

  11:30 - 12:45           DNA damage, p53 and p73 in cell death

                             Chair: Vincenzo De Laurenzi, Cristina Muñoz-Pinedo

Christian Seemayer      FUNCTIONAL P53 RESPONSE IN RASF IN VITRO AND IN VIVO AND INVESTIGATION OF THE APOPTOSIS RESISTANCE AND PROLIFERATION OF RASF IN VITRO

Carine Maisse               Regulation of DeltaNp73: a dominant negative acting isoform.

Tobias Grob                  dNp73 is induced by p53/TAp73 and exerts a dominant negative feedback loop

Eliana Munarriz             FLASH binds to p73 and regulates its function

12:45 - 14:30             Lunch

                              Free Afternoon

  20:30                        Dinner

  Wednesday  February 27^th

8:00 - 9:00                Breakfast

  9:30 - 11:15        Cell death in the nervous system

                          Chair: Huseyin Mehmet, Klaus Schulze-Osthoff

Flavia Troglio                        The neuronal specific adaptor protein Rai (ShcC) inhibits apoptosis triggered by limited neurotrophic support or environmental stress by activating PI3K/Akt

Adeline Lesay                        BCL-2 PROTECTION FROM CYTOCHROME C-INDEPENDENT Apoptosis IN MAPLE SYRUP DISEASE

Jacqueline Beesley                MUTANT PROTEOLIPID PROTEIN TRIGGERS APOPTOSIS IN OLIGODENDROCYTES THROUGH AN ER STRESS PATHWAY INVOLVING CASAPSE-12 ACTIVATION

Huseyin Mehmet                    Induction of Apoptosis or Necrosis in neuronal PC12 cells depending on the redox state of NITRIC OXIDE

Catherine Regnier                  TRAF4 deficiency results in defective neurulation

Pier Giorgio Mastroberardino TG2 ablation reduces neuronal death in a murine model of Huntington's disease

11:15 - 11:45          Coffee break

11:45 - 13:00        Apoptotic signalling

                                  Chair: Klaus Schulze-Osthoff, Huseyin Mehmet

Jiang-yan Yang          The N-terminal caspase-generated RasGAP fragmant protects cells by activating the Ras-PI3K-Akt pathway

Daniela Barilá            REQUIREMENT OF ABL CASPASE CLEAVAGE IN APOPTOTIC SIGNALLING

Mohamed Lamkanfi   TRANSLATION INHIBITION IN APOPTOSIS: CASPASE-DEPENDENT PKR ACTIVATION AND eIF2-a PHOSPHORYLATION

Julieta del Prete        mRNA degradation: a general, early apoptotic event induced by a specific pathway distinct from that leading to DNA degradation

13:00 - 15:00           Lunch at conference site

15:00 - 17:00         Non-caspase proteases in cell death and survival

                                  Chair: Katharina Simon, Marja Jäättelä

Marja Jäättelä                 HSP70 DEPLETION INDUCES CATHEPSIN-MEDIATED TUMOR CELL DEATH AND ERADICATES TUMOR XENOGRAFTS IN IMMUNODEFICIENT MICE

Maria Høyer-Hansen        INVOLVEMENT OF MITOCHONDRIA, ENDOPLASMATIC RETICULUM AND LYSOSOMES IN PROGRAMMED CELL DEATH OF MCF-7 BREAST CANCER CELLS

Nicole Fehrenbacher      TNF-INDUCED DEATH OF MURINE EMBRYONIC FIBROBLASTS BECOMES CATHEPSIN B-DEPENDENT UPON IMMORTALISATION

Michael Bots                    THE SERINE PROTEASE INHIBITOR PI-9/SPI-6 BLOCKS THE PERFORIN/GRANZYME B PATHWAY, WHICH RESULTS IN ESCAPE FROM CTL-MEDIATED CYTOTOXICITY

Ingrid Kolfschoten            IDENTIFICATION AND EXPRESSION OF THE SERINE PROTEASE INHIBITOR SPICI

Lucy Peltenburg             c-Myc-mediated apoptosis in p53-negative human melanoma cells

Diane Bolton                   DEATH OF CD4+ T CELL LINES CAUSED BY HUMAN IMMUNODEFICIENCY VIRUS-1 DOES NOT DEPEND ON CASPASES OR APOPTOSIS

17:00– 17:30           Coffee break                   

17:30 - 19:30         Tumor therapy and counterattack

                       Chair: Katharina Simon, Marja Jäättelä

Mads Daugaard        Specific depletion of hsp70 mrna and protein by small interference rna

Jennifer Rohn             ACTIVATION AND TRAFFICKING OF THE TUMOR-SPECIFIC APOPTOSIS-INDUCING PROTEIN APOPTIN

Alexandra Pietersen   The tumor-specific apoptosis inducing protein Apoptin in a transgenic mouse model

Valentina Screpanti    mISSING- self Recognition bY Perforin-Deficient NK cells

Katharina Simon        Breaking tumour tolerance: Fas ligand induces an anti-tumour response to melanoma antigens

Frederik Igney           Tumor Counterattack: Level and Time-Point of CD95L Expression are not Critical Factors in Mice

Veronica Huber          SECRETION OF FASL-BEARING MICROVESICLES AS A NOVEL MECHANISM OF LYMPHOCYTE APOPTOSIS INDUCED BY TUMOR CELLS

20:00                       Dinner and party

Thursday February 28^th

7:30 - 9:00                Breakfast

                                   Departure